期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 80, 期 16, 页码 4788-4794出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00885-14
关键词
-
资金
- NASA Astrobiology: Exobiology and Evolutionary Biology program [NNX08AO15G]
- NASA Planetary Biology Internship (PBI) program
- NASA Earth and Space Science Fellowship (NESSF) program [13-PLANET13F-0084]
- NASA [95807, NNX08AO15G] Funding Source: Federal RePORTER
Studies of how microorganisms respond to pressure have been limited mostly to the extreme high pressures of the deep sea (i.e., the piezosphere). In contrast, despite the fact that the growth of most bacteria is inhibited at pressures below similar to 2.5 kPa, little is known of microbial responses to low pressure (LP). To study the global LP response, we performed transcription microarrays on Bacillus subtilis cells grown under normal atmospheric pressure (similar to 101 kPa) and a nearly inhibitory LP (5 kPa), equivalent to the pressure found at an altitude of similar to 20 km. Microarray analysis revealed altered levels of 363 transcripts belonging to several global regulons (AbrB, CcpA, CodY, Fur, IolR, ResD, Rok, SigH, Spo0A). Notably, the highest number of upregulated genes, 86, belonged to the SigB-mediated general stress response (GSR) regulon. Upregulation of the GSR by LP was confirmed by monitoring the expression of the SigB-dependent ctc-lacZ reporter fusion. Measuring transcriptome changes resulting from exposure of bacterial cells to LP reveals insights into cellular processes that may respond to LP exposure.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据