期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 81, 期 1, 页码 166-176出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02871-14
关键词
-
资金
- Science Foundation Ireland [SFI/12/ RC/2273]
- HRB postdoctoral fellowship [PDTM/2011/9]
- Health Research Board (HRB) [PDTM-2011-9] Funding Source: Health Research Board (HRB)
Bifidobacterium breve is a common and sometimes very abundant inhabitant of the human gut. Genome sequencing of B. breve JCM 7017 revealed the presence of an extrachromosomal element, designated pMP7017 consisting of>190 kb, thus representing the first reported bifidobacterial megaplasmid. In silico characterization of this element revealed several genomic features supporting a stable establishment of the megaplasmid in its host, illustrated by predicted CRISPR-Cas functions that are known to protect the host against intrusion of foreign DNA. Interestingly, pMP7017 is also predicted to encode a conjugative DNA transfer apparatus and, consistent with this notion, we demonstrate here the conjugal transfer of pMP7017 to representative strains of B. breve and B. longum subsp. longum. We also demonstrate the presence of a megaplasmid with homology to pMP7017 in three B. longum subsp. longum strains.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据