期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 81, 期 4, 页码 1477-1489出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.03290-14
关键词
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资金
- DFG Cluster of Excellence Inflammation at Interfaces and Future Ocean
- Bundesministerium fur Forschung und Bildung, GenoMik Transfer Netzwerk
Two reporter strains were established to identify novel biomolecules interfering with bacterial communication (quorum sensing [ QS]). The basic design of these Escherichia coli-based systems comprises a gene encoding a lethal protein fused to promoters induced in the presence of QS signal molecules. Consequently, these E. coli strains are unable to grow in the presence of the respective QS signal molecules unless a nontoxic QS-interfering compound is present. The first reporter strain designed to detect autoinducer-2 (AI-2)-interfering activities (AI2-QQ. 1) contained the E. coli ccdB lethal gene under the control of the E. coli lsrA promoter. The second reporter strain (AI1-QQ. 1) contained the Vibrio fischeri luxI promoter fused to the ccdB gene to detect interference with acyl-homoserine lactones. Bacteria isolated from the surfaces of several marine eukarya were screened for quorum- quenching (QQ) activities using the established reporter systems AI1-QQ. 1 and AI2-QQ. 1. Out of 34 isolates, two interfered with acylated homoserine lactone (AHL) signaling, five interfered with AI-2 QS signaling, and 10 were demonstrated to interfere with both signal molecules. Open reading frames (ORFs) conferring QQ activity were identified for three selected isolates (Photobacterium sp., Pseudoalteromonas sp., and Vibrio parahaemolyticus). Evaluation of the respective heterologously expressed and purified QQ proteins confirmed their ability to interfere with the AHL and AI-2 signaling processes.
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