4.6 Article

Core Lipopolysaccharide-Specific Phage SSU5 as an Auxiliary Component of a Phage Cocktail for Salmonella Biocontrol

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APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 80, 期 3, 页码 1026-1034

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AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.03494-13

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资金

  1. National Research Foundation of Korea (NRF)
  2. Ministry of Education, Science and Technology [20090078983]
  3. Cooperative Research Program for Agriculture Science & Technology Development, Rural Development Administration, Republic of Korea [PJ009842]
  4. Rural Development Administration (RDA), Republic of Korea [PJ009842012014] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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Salmonella spp. are among the major food-borne pathogens that cause mild diarrhea to severe bacteremia. The use of bacterio-phages to control various food-borne pathogens, including Salmonella, has emerged as a promising alternative to traditional chemotherapy. We isolated the Siphoviridae family phage SSU5, which can infect only rough strains of Salmonella. The blocking of SSU5 adsorption by periodate treatment of host Salmonella cells and spotting and adsorption assays with mutants that contain various truncations in their lipopolysaccharide (LPS) cores revealed that the outer core region of the LPS is a receptor of SSU5. SSU5 could infect O-antigen (O-Ag)-deficient Salmonella mutants that developed by challenging of O-Ag-specific phages, and consequently, it delayed the emergence of the phage-resistant Salmonella population in broth culture when treated together with phages using O-Ag as a receptor. Therefore, these results suggested that phage SSU5 would be a promising auxiliary component of a phage cocktail to control rough strains of Salmonella enterica serovar Typhimurium, which might emerge as resistant mutants upon infection by phages using O-Ag as a receptor.

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