期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 79, 期 22, 页码 7101-7109出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01675-13
关键词
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资金
- National Natural Science Foundation of China [31290232]
- China Ocean Mineral Resources R D Association [DY125-15-T-04]
- China Post-doctoral Science Foundation [2012M520885]
Low-temperature ecosystems represent the largest biosphere on Earth, and yet our understanding of the roles of bacteriophages in these systems is limited. Here, the influence of the cold-active filamentous phage SW1 on the phenotype and gene transcription of its host, Shewanella piezotolerans WP3 (WP3), was investigated by construction of a phage-free strain (WP3 Delta SW1), which was compared with the wild-type strain. The expression of 49 genes, including 16 lateral flagellar genes, was found to be significantly influenced by SW1 at 4 degrees C, as demonstrated by comparative whole-genome microarray analysis. WP3 Delta SW1 was shown to have a higher production of lateral flagella than WP3 and enhanced swarming motility when cultivated on solid agar plates. Besides, SW1 has a remarkable impact on the expression of a variety of host genes in liquid culture, particularly the genes related to the membrane and to the production of lateral flagella. These results suggest that the deep-sea bacterium WP3 might balance the high-energy demands of phage maintenance and swarming motility at low temperatures. The phage SW1 is shown to have a significant influence on the swarming ability of the host and thus may play an important role in adjusting the fitness of the cells in the deep-sea environment.
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