期刊
CHEMISTRY & BIOLOGY
卷 12, 期 8, 页码 941-950出版社
CELL PRESS
DOI: 10.1016/j.chembiol.2005.06.008
关键词
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Directed in vitro evolution can create RNA catalysts for a variety of organic reactions, supporting the RNA world hypothesis, which proposes that metabolic transformations in early life were catalyzed by RNA molecules rather than proteins. Among the most fundamental carbon-carbon bond-forming reactions in nature is the aldol reaction, mainly catalyzed by aldolases that utilize either an enamine mechanism (class I) or a Zn2+ cofactor (class II). We report on isolation of a Zn2+-dependent ribozyme that catalyzes an aldol reaction at its own modified 5' end with a 4300-fold rate enhancement over the uncatalyzed background reaction. The ribozyme can also act as an intermolecular catalyst that transfers a biotinylated benzaldehyde derivative to the aldol donor substrate, coupled to an external hexameric RNA oligonucleotide, supporting the existence of RNA-originated biosynthetic pathways for metabolic sugar precursors and other biomolecules.
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