期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 78, 期 21, 页码 7809-7812出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02122-12
关键词
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资金
- National Major Special Project on New Varieties Cultivation for Transgenic Organisms [2009ZX08009-130B]
- Fundamental Research Funds for the Central Universities of China University of Mining and Technology (Beijing) [2010YH05]
An iterative combinatorial mutagenesis (ICM) strategy was used to engineer deacetoxycephalosporin C synthase of Streptomyces clavuligerus (scDAOCS) for improved activity toward penicillin G. Seven mutational sites were repeatedly combined onto a starter mutant (C155Y Y184H V275I C281Y) of scDAOCS. Eleven improved combinatorial mutants were identified from 24 mutants in four rounds of ICM.
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