期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 78, 期 19, 页码 7137-7140出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01188-12
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资金
- Biotechnology and Biological Sciences Research Council United Kingdom grant [BB/F004753/1]
- BBSRC [BB/F004753/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F004753/1] Funding Source: researchfish
Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.
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