期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 78, 期 11, 页码 4051-4056出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.07994-11
关键词
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资金
- Norwegian-Canadian Holos project
The diversity of protozoan-associated methanogens in cattle was investigated using five universal archaeal small-subunit (SSU) rRNA gene primer sets. Methanobrevibacter spp. and rumen cluster C (distantly related to Thermoplasma spp.) were predominant. Significant differences in species composition among libraries indicate that some primers used previously to characterize rumen methanogens exhibit biased amplification.
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