期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 78, 期 12, 页码 4526-4528出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00348-12
关键词
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资金
- National Science Foundation of China
- Ministry of Science and Technology
- Ministry of Education
- Shanghai Municipality
A genomic cosmid library of Streptomyces clavuligerus was constructed and transferred efficiently by conjugation to Streptomyces lividans, and 12 distinct groups of overlapping cosmid clones that activated the silent actinorhodin biosynthesis gene cluster were identified. This generally applicable high-throughput screening procedure greatly facilitates the identification of antibiotic biosynthesis activators.
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