期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 77, 期 10, 页码 3197-3201出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00151-11
关键词
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资金
- Major Basic Research Development Program of China [2007CB714303]
- National Natural Science Foundation of China [31000014, 31070062]
Production of N-acetyl-D-neuraminic acid (Neu5Ac) via biocatalysis is traditionally conducted using isolated enzymes or whole cells. The use of isolated enzymes is restricted by the time-consuming purification process, whereas the application of whole cells is limited by the permeability barrier presented by the microbial cell membrane. In this study, a novel type of biocatalyst, Neu5Ac aldolase presented on the surface of Bacillus subtilis spores, was used for the production of Neu5Ac. Under optimal conditions, Neu5Ac at a high concentration (54.7 g liter(-1)) and a high yield (90.2%) was obtained under a 5-fold excess of pyruvate over N-acetyl-D-mannosamine. The novel biocatalyst system, which is able to express and immobilize the target enzyme simultaneously on the surface of B. subtilis spores, represents a suitable alternative for value-added chemical production.
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