期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 78, 期 2, 页码 481-492出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.06159-11
关键词
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资金
- Comite Nord Plants de Pommes de terre (CNPPT)
- Association Nationale de la Recherche et de la Technologie [CIFRE 2007/0117]
- Centre National de la Recherche Scientifique (CNRS)
- Agence Nationale de la Recherche (ANR)
- Region Ile-de-France (IUDS)
- Programme National d'Ecotoxicologie (PNETOX)
- Programme Ecosphere Continentale et Cotiere (EC2CO)
Degradation of the quorum-sensing (QS) signals known as N-acylhomoserine lactones (AHL) by soil bacteria may be useful as a beneficial trait for protecting crops, such as potato plants, against the worldwide pathogen Pectobacterium. In this work, analytical chemistry and microbial and molecular approaches were combined to explore and compare biostimulation of native and introduced AHL-degrading Rhodococcus erythropolis populations in the rhizosphere of potato plants cultivated in farm greenhouses under hydroponic conditions. We first identified gamma-heptalactone (GHL) as a novel biostimulating agent that efficiently promotes plant root colonization by AHL-degrading R. erythropolis population. We also characterized an AHLdegrading biocontrol R. erythropolis isolate, R138, which was introduced in the potato rhizosphere. Moreover, root colonization by AHL-degrading bacteria receiving different combinations of GHL and R138 treatments was compared by using a cultivationbased app roach (percentage of AHL-degrading bacteria), pyrosequencing of PCR-amplified rrs loci (total bacterial community), and quantitative PCR (qPCR) of the qsdA gene, which encodes an AHL lactonase in R. erythropolis. Higher densities of the AHLdegrading R. erythropolis population in the rhizosphere were observed when GHL treatment was associated with biocontrol strain R138. Under this condition, the introduced R. erythropolis population displaced the native R. erythropolis population. Finally, chemical analyses revealed that GHL, gamma-caprolactone (GCL), and their by-products, gamma-hydroxyheptanoic acid and gamma-hydroxycaproic acid, rapidly disappeared from the rhizosphere and did not accumulate in plant tissues. This integrative study highlights biostimulation as a potential innovative approach for improving root colonization by beneficial bacteria.
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