pmoA Primers for Detection of Anaerobic Methanotrophs

Published pmoA primers do not match the pmoA sequence of Candidatus Methylomirabilis oxyfera, a bacterium that performs nitrite-dependent anaerobic methane oxidation. Therefore, new pmoA primers for the detection of Ca. Methylomirabilis oxyfera-like methanotrophs were developed and successfully tested on freshwater samples from different habitats. These primers expand existing molecular tools for the study of methanotrophs in the environment.