期刊
PEPTIDES
卷 26, 期 8, 页码 1492-1496出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2005.03.016
关键词
nociceptin/orphanin FQ; NOP receptor; human bronchus; cholinergic neurotransmission; electrical field stimulation; opioids; airways
Nociceptin/orphanin FQ (N/OFQ) has been reported to inhibit neurogenic contractions in various tissues, including guinea pig airways. In the present study, we investigated the ability of N/OFQ to affect cholinergic contractions of human bronchi elicited by electrical field stimulation (EFS). Tissues were obtained from 23 patients undergoing surgery for lung cancer. EFS (20 Hz, 320 mA, 1.5 ms, 10 s) was applied five times every 20 min. Contractions induced by EFS were abolished by either TTX (1 mu M) or atropine (1 mu M) and concentration-dependently (10 nM-1 mu M) inhibited by N/OFQ (E-max, 11.5 +/- 1.8% inhibition). The inhibitory effects of N/OFQ were mimicked by the N/OFQ receptor (NOP) ligand [Arg(14), Lys(15)]N/OFQ which displayed however, higher significant maximal effects (17.7 +/- 2.9% inhibition, P < 0.05). The actions of N/OFQ and [Arg(14), Lys(15)]N/OFQ were not affected by naloxone (1 mu M) while prevented by the selective NOP receptor antagonist UFP-101 (10 mu M). Moreover, the inhibitory effects of NOP agonists were no longer evident in tissues treated with tertiapin (10 mu M), an inhibitor of inward-rectifier potassium channels. In conclusion, the present data demonstrate that N/OFQ inhibited acetylcholine (ACh) release in the human bronchi via NOP receptor activation. This effect may involve stimulation of potassium currents. (c) 2005 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据