期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 76, 期 6, 页码 1783-1788出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00668-09
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资金
- New Energy and Industrial Technology Development Organization (NEDO) [P05032]
Microbiologically influenced corrosion of steel in anaerobic environments has been attributed to hydrogenotrophic microorganisms. A sludge sample collected from the bottom plate of a crude-oil storage tank was used to inoculate a medium containing iron (Fe-0) granules, which was then incubated anaerobically at 37 degrees C under an N-2-CO2 atmosphere to enrich for microorganisms capable of using iron as the sole source of electrons. A methanogen, designated strain KA1, was isolated from the enrichment culture. An analysis of its 16S rRNA gene sequence revealed that strain KA1 is a Methanococcus maripaludis strain. Strain KA1 produced methane and oxidized iron much faster than did the type strain of M. maripaludis, strain JJ(T), which produced methane at a rate expected from the abiotic H-2 production rate from iron. Scanning electron micrographs of iron coupons that had been immersed in either a KA1 culture, a JJ(T) culture, or an aseptic medium showed that only coupons from the KA1 culture had corroded substantially, and these were covered with crystalline deposits that consisted mainly of FeCO3.
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