期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 76, 期 19, 页码 6701-6705出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00361-10
关键词
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资金
- National Basic Research Program (973) of China [2011CB100805]
- State Key Laboratory of Animal Nutrition [2004DA125184(tuan)0801]
One clone encoding glycoside hydrolases was identified through functional screening of a rumen bacterial artificial chromosome (BAC) library. Of the 68 open reading frames (ORFs) predicted, one ORF encodes a novel endo-beta-1,4-xylanase with two catalytic domains of family GH43 and two cellulose-binding modules (CBMs) of family IV. Partial characterization showed that this endo-xylanase has a greater specific activity than a number of other xylanases over a wide temperature range at neutral pH and could be useful in some industrial applications.
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