Detection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by 15N and 13C Incorporation

2,4,6-Trinitrotoluene (N-15 or C-13 labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [N-15] TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [C-13] TNT cultures and was related to Lysobacter taiwanensis.