期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 76, 期 9, 页码 2997-3003出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02661-09
关键词
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资金
- Chemical Sciences, Geosciences and Biosciences Division of the Office of Basic Energy Biosciences [DE-FG02-08ER15976]
- U.S. Department of Energy Joint Genome Institute
- Gordon and Betty Moore Foundation/Marine Microbiology Initiative
- Office of Science (BER), U.S. Department of Energy [DE-FG02-97ER62452, DE-FG02-97ER62454]
- National Science Foundation
- U.S. Department of Energy (DOE) [DE-FG02-08ER15976] Funding Source: U.S. Department of Energy (DOE)
Culture-independent studies have indicated that there is significant diversity in the ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) enzymes used by marine, freshwater, and terrestrial autotrophic bacteria. Surprisingly, little is known about the catalytic properties of many environmentally significant RubisCO enzymes. Because one of the goals of RubisCO research is to somehow modify or select for RubisCO molecules with improved kinetic properties, a facile means to isolate functional and novel RubisCO molecules directly from the environment was developed. In this report, we describe the first example of functional RubisCO proteins obtained from genes cloned and characterized from metagenomic libraries derived from DNA isolated from environmental samples. Two form IA marine RubisCO genes were cloned, and each gene supported both photoheterotrophic and photoautotrophic growth of a RubisCO deletion strain of Rhodobacter capsulatus, strain SBI/II-, indicating that catalytically active recombinant RubisCO was synthesized. The catalytic properties of the metagenomic RubisCO molecules were further characterized. These experiments demonstrated the feasibility of studying the functional diversity and enzymatic properties of RubisCO enzymes without first cultivating the host organisms. Further, this proof of concept experiment opens the way for development of a simple functional screen to examine the properties of diverse RubisCO genes isolated from any environment, and subsequent further bioselection may be possible if the growth conditions of complemented R. capsulatus strain SBI/II- are varied.
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