期刊
INTERNATIONAL IMMUNOLOGY
卷 17, 期 8, 页码 983-991出版社
OXFORD UNIV PRESS
DOI: 10.1093/intimm/dxh279
关键词
aging; cellular activation; glycosylation; immunosenescence; signal transduction
类别
资金
- NIA NIH HHS [AG00114, AG19619] Funding Source: Medline
Many of the downstream signaling defects observed in aged T cells are believed to be the result of very early events involving the initial interaction between T cells and antigen-presenting cells. Recent findings suggest that this interaction is hindered by glycosylated surface macromolecules, including CD43, on the T cell surface. Treatment of CD4(+) T cells by O-sialoglycoprotein endopeptidase (OSGE), which cleaves glycosylated forms of CD43, restores the ability of cells from aged mice to form immunological synapses and to express early activation markers. Here we show that OSGE enhances Ca2+ influx in T cells from CB6F(1) mice, and enhances their ability to produce IL-2, IL-4, IL-5, IL-6, IL-10, IL-13 and IFN gamma at the mRNA level, and IL-2 and IFN gamma at the protein level, in the first 6 h after activation. Although OSGE has little effect on synapse formation in CD4(+) T cells from young mice, our new data show that OSGE increases the production of most cytokines by young as well as old T cells. Secretion of the T(h)2 cytokine, IL-4, was altered only slightly by OSGE treatment, suggesting that the removal of OSGE-sensitive surface molecules may have differential effects on T(h)1 and T(h)2 cytokines. These data support a model in which O-glycosylated surface proteins inhibit CD4(+) lymphocyte activation in both young and old mice, and in which such glycoproteins contribute to the age-related decline in cytokine production.
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