期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 75, 期 20, 页码 6613-6615出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01587-09
关键词
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资金
- MRC-
- EPSRC
- BBSRC-
- MoD-funded Bionanotechnology Interdisciplinary Research Collaboration
- BBSRC
We have developed a stable isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacI(q) gene from pYanni3 and the lac promoter, P-A1/04/03, from pJBA24.
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