期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 75, 期 9, 页码 2720-2726出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02738-08
关键词
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资金
- U.S. Department of Agriculture, Agricultural Research Service
- NBS-ERC (Nano Bioelectronics and Systems Research Center)/KOSEF (Korea Science and Engineering Foundation)
Although chemically defined media have been developed and widely used to study the expression of virulence factors in the model plant pathogen Pseudomonas syringae, it has been difficult to link specific medium components to the induction response. Using a chemostat system, we found that iron is the limiting nutrient for growth in the standard hrp-inducing minimal medium and plays an important role in inducing several virulence-related genes in Pseudomonas syringae pv. tomato DC3000. With various concentrations of iron oxalate, growth was found to follow Monod-type kinetics for low to moderate iron concentrations. Observable toxicity due to iron began at 400 mu M Fe3+. The kinetics of virulence factor gene induction can be expressed mathematically in terms of supplemented-iron concentration. We conclude that studies of induction of virulence-related genes in P. syringae should control iron levels carefully to reduce variations in the availability of this essential nutrient.
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