4.6 Article

Optimization of RNA Extraction for PCR Quantification of Aromatic Compound Degradation Genes

期刊

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 76, 期 4, 页码 1282-1284

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AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01939-09

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  1. National Science Foundation [0302645]
  2. Directorate For Engineering
  3. Div Of Chem, Bioeng, Env, & Transp Sys [0302645] Funding Source: National Science Foundation

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Seven different bacterial strains and primer sets and a mixed community were used to evaluate the use of reverse transcriptase quantitative PCR (Q-PCR) and Q-PCR of oxygenase genes to assess various approaches for monitoring the bioremediation of polluted sites. Differences in maximum activity were seen when different RNA extraction kits were compared.

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