期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 75, 期 9, 页码 2940-2944出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01333-08
关键词
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资金
- Environmental Division of the California Department of Transportation [43A0168, TO 23]
- Cooperative Institute for Coastal and Estuarine Environmental Technology (CICEET) [07-043, 111A81]
Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 mu M PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.
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