期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 75, 期 12, 页码 4206-4210出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02906-08
关键词
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资金
- German Research Foundation [SPP 1104]
- German Federal Ministry of Education and Research
To develop an expression system for the magnetotactic bacterium Magnetospirillum gryphiswaldense, we compared gene expression from the widely used Escherichia coli P-lac promoter with that from known and predicted genuine M. gryphiswaldense promoters. With the use of green fluorescent protein as a reporter, the highest expression level was observed with the magnetosomal P-mamDC promoter. We demonstrate that this promoter can be used for the expression of modified magnetosome proteins to generate antibody-binding magnetosomes.
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