期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 76, 期 1, 页码 243-253出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01059-09
关键词
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资金
- Rochester University School of Medicine and Dentistry (United States) [pLP170, pPCS223, pPCS1001, pLPR1, pPCS1002]
- Department of Molecular Biotechnology, Hiroshima University, Hiroshima, Japan [pQF50, pbeta01, pbeta02, pbeta03]
- Ohio State University [pAL101, pAL102, pAL105, pAL106]
- Institute for Medical Immunology from the Universite Libre de Bruxelles
Quorum-sensing (QS) regulates the production of key virulence factors in Pseudomonas aeruginosa and other important pathogenic bacteria. In this report, extracts of leaves and bark of Combretum albiflorum (Tul.) Jongkind (Combretaceae) were found to quench the production of QS-dependent factors in P. aeruginosa PAO1. Chromatographic fractionation of the crude active extract generated several active fractions containing flavonoids, as shown by their typical spectral features. Purification and structural characterization of one of the active compounds led to the identification of the flavan-3-ol catechin [(2R, 3S)-2-(3,4-dihydroxyphenyl)-3,4-dihydro-1(2H)-benzopyran-3,5,7-triol]. The identity of catechin as one of the active molecules was confirmed by comparing the high-pressure liquid chromatography profiles and the mass spectrometry spectra obtained for a catechin standard and for the active C. albiflorum fraction. Moreover, standard catechin had a significant negative effect on pyocyanin and elastase productions and biofilm formation, as well as on the expression of the QS-regulated genes lasB and rhlA and of the key QS regulatory genes lasI, lasR, rhlI, and rhlR. The use of RhlR- and LasR-based biosensors indicated that catechin might interfere with the perception of the QS signal N-butanoyl-L-homoserine lactone by RhlR, thereby leading to a reduction of the production of QS factors. Hence, catechin, along with other flavonoids produced by higher plants, might constitute a first line of defense against pathogenic attacks by affecting QS mechanisms and thereby virulence factor production.
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