期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 75, 期 7, 页码 2139-2147出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02352-08
关键词
-
资金
- Agriculture and Agri-Food Canada Pest Management Centre Pesticide Risk Reduction
- Minor Use programs' Improved Farming Systems and Practices
- Natural Science and Engineering Research Council of Canada (NSERC)
The first complete genome sequence for a myoviridal bacteriophage, Phi Ea21-4, infecting Erwinia amylovora, Erwinia pyrifoliae, and Pantoea agglomerans strains has been determined. The unique sequence of this terminally redundant, circularly permuted genome is 84,576 bp. The Phi Ea21-4 genome has a GC content of 43.8% and contains 117 putative protein-coding genes and 26 tRNA genes. Phi Ea21-4 is the first phage in which a precisely conserved rho-independent terminator has been found dispersed throughout the genome, with 24 copies in all. Also notable in the Phi Ea21-4 genome are the presence of tRNAs with six- and nine-base anticodon loops, the absence of a small packaging terminase subunit, and the presence of nadV, a principle component of the NAD(+) salvage pathway, which has been found in only a few phage genomes to date. Phi Ea21-4 is the first reported Felix O1-like phage genome; 56% of the predicted Phi Ea21-4 proteins share homology with those of the Salmonella phage. Apart from this similarity to Felix O1, the Phi Ea21-4 genome appears to be substantially different, both globally and locally, from previously reported sequences. A total of 43 of the 117 genes are unique to Phi Ea21-4, and 32 of the Felix O1-like genes do not appear in any phage genome sequences other than Phi Ea21-4 and Felix O1. N-terminal sequencing and matrix-assisted laser desorption ionization-time of flight analysis resulted in the identification of five Phi Ea21-4 genes coding for virion structural proteins, including the major capsid protein.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据