期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 75, 期 15, 页码 5153-5156出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00629-09
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资金
- Horticulture Australia Ltd.
- Orica Australia Ltd.
In silico substrate docking of both stereoisomers of the pesticide chlorfenvinphos (CVP) in the phosphotriesterase from Agrobacterium radiobacter identified two residues (F131 and W132) that prevent productive substrate binding and cause stereospecificity. A variant (W131H/F132A) was designed that exhibited ca. 480-fold and 8-fold increases in the rate of Z-CVP and E-CVP hydrolysis, respectively, eliminating stereospecificity.
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