期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 75, 期 24, 页码 7850-7854出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01532-09
关键词
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资金
- MICINN [BIO2007-61194, EUI2008-03610]
- BBSRC [BB/C504735/1]
- CIBER de Bioingenier a, Biomateriales y Nanomedicina (CIBER-BBN), Spain
- MEC, Spain
- ICREA ACADEMIA award (Catalonia, Spain)
Coproduction of DnaK/DnaJ in Escherichia coli enhances solubility but promotes proteolytic degradation of their substrates, minimizing the yield of unstable polypeptides. Higher eukaryotes have orthologs of DnaK/DnaJ but lack the linked bacterial proteolytic system. By coexpression of DnaK and DnaJ in insect cells with inherently misfolding-prone recombinant proteins, we demonstrate simultaneous improvement of soluble protein yield and quality and proteolytic stability. Thus, undesired side effects of bacterial folding modulators can be avoided by appropriate rehosting in heterologous cell expression systems.
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