期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 74, 期 13, 页码 4226-4230出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00013-08
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We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. The data demonstrate that IMS combined with real-time RT-PCR will be useful as a rapid and sensitive method for detecting food-borne microbial contaminants.
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