期刊
DEVELOPMENTAL DYNAMICS
卷 233, 期 4, 页码 1488-1495出版社
WILEY
DOI: 10.1002/dvdy.20456
关键词
osteopontin; matricellular; testis; SIBLING; gonad; bone sialoprotein; dentin matrix protein
资金
- NCRR NIH HHS [RR 15555-04S1] Funding Source: Medline
- NHLBI NIH HHS [HL070865-01A1] Funding Source: Medline
Matrix proteins play important roles in tissue morphogenesis. We have studied the expression of genes encoding the related SIBLING glycoproteins osteopontin (OPN), bone sialoprotein (BSP), and dentin matrix protein (DMP) during the development of male and female gonads during mouse embryogenesis. Opn mRNA was expressed specifically by Sertoli cells of the developing testis cords, in the mesonephric tubules of both sexes, and, transiently, in the Mullerian ducts of both sexes, as determined by whole-mount and section in situ hybridization. OPN protein was detected in the cytoplasm of Sertoli cells and luminal cells of the mesonephric tubules, with small amounts associated with the plasma membrane of germ cells. We found no defects in developing testes of Opn-/- mice using a range of cell type-specific markers, suggesting that other SIBLING proteins may function in testis development. Dmp and Bsp mRNA was also expressed in the developing testis cords, supporting the view that all three SIBLING proteins may contribute to testis differentiation. (c) 2005 Wiley-Liss, Inc.
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