期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 74, 期 13, 页码 4133-4143出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00454-08
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资金
- NIAID NIH HHS [R01 AI 036596, R01 AI036596] Funding Source: Medline
Marine sponges in the genus Ircinia are known to be good sources of secondary metabolites with biological activities. A major obstacle in the development of sponge-derived metabolites is the difficulty in ensuring an economic, sustainable supply of the metabolites. A promising strategy is the ex situ culture of sponges in closed or semiclosed aquaculture systems. In this study, the marine sponge Ircinia strobilina (order Dictyoceratida: family Irciniidae) was collected from the wild and maintained for a year in a recirculating aquaculture system. Microbiological and molecular community analyses were performed on freshly collected sponges and sponges maintained in aquaculture for 3 months and 9 months. Chemical analyses were performed on wild collected. sponges and individuals maintained in aquaculture for 3 months and I year. Denaturing gradient gel electrophoresis was used to assess the complexity of and to monitor changes in the microbial communities associated with L strobilina. Culture-based and molecular techniques showed an increase in the Bacteroidetes and Alpha- and Gaminaproteobacteria components of the bacterial community in aquaculture. Populations affiliated with Beta- and Deltaproteobacteria, Clostridia, and Planctomycetes emerged in sponges maintained in aquaculture. The diversity of bacterial communities increased upon transfer into aquaculture.
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