期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 99, 期 8, 页码 1685-1691出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2005.05.012
关键词
ruthenium complex; DNA; spectroscopy
Two new ruthenium(II) complexes of [Ru(bpy)(pp[2,3]p)(2)](ClO4)(2) and [Ru(phen)(pp[2,3]p)(2)](ClO4)(2) (bpy = 2,2'-bipyridine, phen = 1,10-phenanthroline, pp[2,3]p = pyrido[2',3':5,6]pyrazino[2,3-f][1,10]phenanthroline) have been synthesized and characterized by elemental analysis and H-1 NMR spectra. The calf thymus DNA-binding properties of the two complexes were investigated by UV-visible and emission spectroscopy, competitive binding experiments with ethidium bromide and viscosity measurements. The results indicate that the two complexes intercalate between the base pairs of the DNA tightly with intrinsic DNA-binding constants of 3.08 x 10(6) and 6.53 x 10(6) M-1 in buffered 50 mM NaCl, respectively, which are much larger than 6.9 x 10(5) M-1 for [Ru(bpy)(2)(pp[2,3]p)](ClO4)(2) containing two ancillary ligands of bpy. (c) 2005 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据