期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 74, 期 22, 页码 7059-7063出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01330-08
关键词
-
资金
- National Science Foundation (NSF) [MCB-0347317]
- National Institutes of Health (NIH) [AI50661]
- NSF Research Experience for Undergraduates site award [DBI-0453353]
- W. M. Keck Foundation
- [pRZ5412]
We have developed a transposon mutagenesis system for Vibrio fischeri ES114 that utilizes a hyperactive mutant Tn5 transposase (E54K and M56A) and optimized transposon ends. Using a conjugation-based procedure, we obtained independent single-insertion mini-Tn5 mutants at a rate of similar to 10(-6). This simple and inexpensive technique represents a significant improvement over previous methods for transposon mutagenesis of V. fischeri and should be applicable to many other bacteria.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据