期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 75, 期 3, 页码 608-617出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01183-08
关键词
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资金
- EU [EVK4-CT2000-00028]
- Italian Ministry of University and Research [PRIN 2001, 2003]
- Italian Ministry of Foreign Affairs
Six Leptolyngbya strains, isolated from the archaeological surfaces of hypogean sites, were phenotypically and genetically characterized by light and electron microscopy and 16S rRNA gene and 16S-23S internally transcribed spacer (ITS) sequencing. Three phycoerythrin-rich (red) and three phycocyanin-rich (green) isolates were assigned to different operational taxonomic units (OTUs). Among the green isolates, one strain showed an OTU intraspecific variation due to differences in the ITS sequences and genomic polymorphism. Within the ITS sequence, variable regions, conserved domains and tRNAIle and tRNAAla genes showed high sequence identity among the phylotypes. Together, these data indicated a relatedness of the six strains to other Leptolyngbya from subaerophytic and geothermal environments and allowed the definition of novel Leptolyngbya OTUs.
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