期刊
CHEMICAL PHYSICS
卷 315, 期 1-2, 页码 142-154出版社
ELSEVIER
DOI: 10.1016/j.chemphys.2005.04.008
关键词
BLUF domain; AppA; blue-light photoreceptor; Rhodobacter sphaeroides; absorption spectroscopy; fluorescence spectroscopy; fluorescence up-conversion; photo-cycle; flavoprotein; FAD; FMN; riboflavin
The BLUF domain of the transcriptional anti-repressor protein AppA from the non-sulfur anoxyphototrophic purple bacterium Rhodobacter sphaeroides was characterized by absorption and emission spectroscopy. The BLUF domain constructs AppA] 48 (consisting of amino-acid residues 1-148) and AppA(126) (amino-acid residues 1-126) are investigated. The cofactor of the investigated domains is found to consist of a mixture of the flavins riboflavin, FMN, and FAD. The dark-adapted domains exist in two different active receptor conformations (receptor states) with different sub-nanosecond fluorescence lifetimes (BLUFr,f and BLUFr,sl) and a small non-interacting conformation (BLUF,,,). The active receptor conformations are transformed to putative signalling states (BLUFs,f and BLUFs,sl) of low fluorescence efficiency and picosecond fluorescence lifetime by blue-light excitation (light-adapted domains). In the dark at room temperature both signalling states recover back to the initial receptor states with a time constant of about 17 min. A quantum yield of signalling state formation of about 25% was determined by intensity dependent transmission measurements. A photo-cycle scheme is presented including photo-induced charge transfer complex formation, charge recombination, and protein binding pocket reorganisation. (C) 2005 Elsevier B.V. All rights reserved.
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