Synthetic yeast oligomannosides as biological probes:: α-D-Manp (1→3) α-D-Manp (1→2) α-D-Manp and α-D-Manp (1→3) α-D-Manp (1→2) α-D-Manp (1→2) α-D-Manp as Crohn's disease markers

The anti-Saccharomyces cerevisiae antibodies (ASCA) are markers for Crohn's disease used for diagnostic, phenotypic characterization and sero-epidermiological studies. Antibody detection is made by different immunoenzymatic tests using S. cerevisiae mannans which are both complex and poorly standardized antigens. Here we construct the major discriminating epitopes comprised within this antigen. When coupled to linker arm and a peptidic carrier to functionalize microtiter plates, they were able to discriminate serological responses between Crohn's disease and ulcerative colitis, another form of inflammatory bowel disease. (c) 2005 Elsevier Ltd. All rights reserved.