期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 102, 期 32, 页码 11504-11509出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0500848102
关键词
confocal microscopy; female reproductive tissue; HIV transmission; organ culture; tissue surface reconstruction
资金
- NIAID NIH HHS [AI 49806] Funding Source: Medline
- NIDCR NIH HHS [R21 DE015090, DE 15090] Funding Source: Medline
- NIDDK NIH HHS [R24 DK064400, DK 64400] Funding Source: Medline
We have developed human cervicovaginal organ culture systems to examine the initiating events in HIV transmission after exposure to various sources of HIV infectivity, including semen. Newly infected cells were detected in the cervical submucosa 3-4 days after exposure to a primary HIV isolate. At earlier times, extensive and stable binding occurred when cervical surfaces were exposed to virions or seminal cells. Cervical mucus provided some protection for the endocervical surface, by physically trapping virions and seminal cells. Confocal microscopy combined with 3D surface reconstruction revealed that virions could both bind to the external surface of the cervical epithelium and actually penetrate beneath the epithelial surface. In quantitative assays, pretreatment with a blocking antibody directed against 1131 integrin reduced HIV virion binding. Collectively, these results highlight a continuum of complex interactions that occurs when natural sources of HIV infectivity are deposited onto mucosal surfaces in the female reproductive tract.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据