期刊
JOURNAL OF NEUROSCIENCE
卷 25, 期 32, 页码 7342-7351出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4603-04.2005
关键词
addiction; AMPA receptor; dopamine; LTP; plasticity; protein kinase A
资金
- NIDA NIH HHS [R01 DA015835, DA00453, R37 DA015835, K02 DA000453, DA015835] Funding Source: Medline
Addiction is believed to involve glutamate-dependent forms of synaptic plasticity that promote the formation of new habits focused on drug seeking. We used primary cultures of rat prefrontal cortex (PFC) neurons to explore mechanisms by which dopamine-releasing psychomotor stimulants such as cocaine and amphetamine influence synaptic plasticity, focusing on AMPA receptor trafficking because of its key role in long-term potentiation (LTP). Brief stimulation of D-1 dopamine receptors increased surface expression of glutamate receptor 1 (GluR1)-containing AMPA receptors through a protein kinase A-dependent mechanism, by increasing their rate of externalization at extrasynaptic sites. Newly externalized GluR1 remained extrasynaptic under basal conditions but could be translocated into synapses by subsequent NMDA receptor activation. These results suggest that D-1 receptors may facilitate LTP by increasing the AMPA receptor pool available for synaptic insertion. However, stimulation of D-2 receptors decreased surface and synaptic GluR1 expression. These findings are discussed in the context of evidence that D-1 and D-2 receptors act independently rather than antagonistically in the intact PFC. D-1 receptor facilitation of AMPA receptor synaptic insertion helps explain D-1 receptor-dependent facilitation of LTP and learning in the normal brain. Abnormal engagement of this mechanism during unregulated dopamine release may account for maladaptive plasticity after repeated exposure to cocaine or amphetamine.
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