期刊
JOURNAL OF NEUROSCIENCE
卷 25, 期 32, 页码 7324-7332出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2042-05.2005
关键词
chromaffin; catecholamine; neuropeptide; exocytosis; endocytosis; trafficking; stress
资金
- NHLBI NIH HHS [T32HL007887, T32HL07653, T32 HL007887, T32 HL007653] Funding Source: Medline
- NINDS NIH HHS [R01 NS052123, 1R01NS052123] Funding Source: Medline
Chromaffin cells of the adrenal medulla are a primary neuroendocrine output of the sympathetic nervous system. When stimulated, they secrete a host of transmitter molecules, including catecholamines and neuropeptides, through the fusion of dense core secretory granules with the cell surface. At basal firing rates, set by the sympathetic tone, chromaffin cells selectively release catecholamines at a modest rate. Stress-mediated sympathetic activation leads to elevated catecholamine secretion and also evokes neuropeptide release. Catecholamines and neuropeptides are copackaged in the same granules; thus, it is unclear how this activity-dependent differential transmitter release is achieved. In this report, we use electrophysiological, electrochemical, fluorescence, and immunocytochemical approaches to quantify transmitter release under physiological electrical stimulation at the single cell level. We provide data to show that chromaffin cells selectively release catecholamine under basal firing conditions but release both neuropeptides and catecholamines under conditions that match acute stress. We further show that this differential transmitter release is achieved through a regulated activity-dependent dilation of the granule fusion pore. Thus, chromaffin cells may regulate release of different transmitters through a simple size-exclusion mechanism.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据