期刊
REGULATORY PEPTIDES
卷 130, 期 1-2, 页码 27-34出版社
ELSEVIER
DOI: 10.1016/j.regpep.2005.03.006
关键词
adrenomedullin; rat cerebral endothelial cell; hydrogen peroxide; permeability; viability
资金
- NHLBI NIH HHS [HL-50587, HL-46558, HL-30260] Funding Source: Medline
- NIDDK NIH HHS [DK-62372] Funding Source: Medline
Increased permeability and reduced cerebral endothelial cell (CEC) viability induced by oxidative stress are the hallmarks of the blood-brain barrier disruption. In Our experiments hydrogen peroxide (H2O2, 0.5 mM) induced a continuous decrease of the transendothelial electrical resistance (TEER) and resulted in intercellular gap formations in cultured rat CECs. Adrenomedullin (AM) increased TEER, enhanced peripheral localization of F-actin hands and attenuated the increased permeability induced by H2O2. Furthermore, AM treatment preserved mitochondrial membrane potential, attenuated cytochrome c release, and consequently improved CEC viability in H2O2 treated cultures. These results suggest that AM treatment protects CECs against oxidative injury. (c) 2005 Elsevier B.V. All rights reserved.
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