期刊
EXPERIMENTAL CELL RESEARCH
卷 308, 期 2, 页码 357-363出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2005.05.011
关键词
beta-catenin; lymphocyte enhancer factor-1; nuclear localization signal
Nuclear accumulation of beta-catenin plays an important role in the Writ signaling pathway. In the nucleus, beta-catenin acts as a transcriptional co-activator for TCF/LEF family of transcription factors. It has been shown that lef-1 contains a typical basic type nuclear localization signal (NLS) and is transported into the nucleus by the conventional import pathway. In this study, we found that a mutant lef-1 lacking the classical NLS accumulated in the nucleus of living cells, when beta-catenin was co-expressed. In addition, in a cell-free import assay, lef-1 migrated into the nucleus in the presence of beta-catenin alone without any other soluble factors. In contrast, another mutant lef-1 lacking the beta-catenin binding domain failed to migrate into the nucleus, even in the presence of beta-catenin. These findings indicate that beta-catenin atone can mediate the nuclear import of lef-1 through the direct binding. Collectively, we propose that there are two distinct pathways for the nuclear import of lef-1: importin alpha/beta-mediated and beta-catenin-mediated one, which provides a novel paradigm for Writ signaling pathway. (c) 2005 Elsevier Inc. All rights reserved.
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