期刊
SCIENCE
卷 309, 期 5738, 页码 1210-1215出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1112064
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资金
- NIGMS NIH HHS [GM28470, GM57510] Funding Source: Medline
The structure of a Synaptic intermediate of the site-specific recombinase gamma delta resolvase covalently linked through Ser(10) to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA. Because the two cleaved DNA duplexes that are to be recombined lie on opposite sides of the core tetramer, large movements of both protein and DNA are required to achieve strand exchange. The two dimers linked to the DNAs that are to be recombined are held together by a flat interface. This may allow a 180 degrees rotation of one dimer relative to the other in order to reposition the DNA duplexes for strand exchange.
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