期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 91, 期 4, 页码 468-473出版社
WILEY
DOI: 10.1002/bit.20525
关键词
protein biosynthesis; poly(A) leader in mRNA; cell-free translation systems; translational enhancers; continuous-exchange cell-free (CECF) systems
Poly(A) sequence of 25 adenylic residues placed immediately before the start codons of the green fluorescent protein (GFP) and firefly luciferase (Luc) mRNAs is shown to provide a high rate of translation of the heterologous messages in eukaryotic cell-free translation systems. Also the poly(A) leader is found to provide the abolition of the inhibition of translation at excess mRNA concentrations. The possibility of the practical use of the constructs with the poly(A) leader for preparative protein production is demonstrated in the wheat germ continuous-exchange cell-free (CECF) translation system. (c) 2005 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据