期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1085, 期 1, 页码 86-90出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2005.05.043
关键词
quaternary ammonium compounds; surfactants; alkyltrimethylammonium; suppressed conductivity; UV; lysing reagents
A method has been developed which allows simultaneous determination of three linear alkyl trimethylammonium salts. Dodecyltrimethylammonium chloride, tetradecyltrimethylammonium bromide and hexadecyltrimethylammonium chloride are widely used as main active ingredients of lysing reagents for blood cell analyzers which perform white blood cells differential determination into two or more subpopulations by impedance analysis. The ion-pair on styrene-divinyl benzene chromatographic phase looks like a suitable, reliable and long term stable tool for separation of such quaternary compounds. The detection based on suppressed conductivity was chosen because of the lack of significance chromophores. A micromembrane suppressor device compatible with high solvent concentration (up to 80%) was used in order to minimize the conductivity background before the detection. In the present work we show how the chemical post column derivatization makes the alkyl chain detectable also by UV direct detection at 210 nm. (c) 2005 Elsevier B.V. All rights reserved.
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