期刊
JOURNAL OF NEUROSCIENCE
卷 25, 期 35, 页码 8104-8111出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2611-05.2005
关键词
neuromuscular; channel block; cholinergic; quantal analysis; patch clamp; spinal cord; K channel
资金
- NINDS NIH HHS [R37 NS018205, NS18205, R01 NS018205] Funding Source: Medline
The transparent spinal cord and electrically compact fast muscle of zebrafish offer the first opportunity to perform simultaneous whole-cell patch-clamp recordings from both motor neuron and target skeletal muscle in situ. Our paired recordings reveal the fastest reported kinetics for both spontaneous and evoked synaptic currents at any synapse and a large quantal size that facilitates the resolution of spontaneous synaptic currents. We used this preparation to test the recent proposal that open channel block of the acetylcholine receptor by acetylcholine modulates the kinetics and timing of transmission between nerve and muscle in larval zebrafish (Legendre et al., 2000). Contrary to the predictions of this model, we find similar delay and onset kinetics of synaptic current at positive and negative muscle membrane potentials, even after inhibition of acetylcholinesterase. In contrast, blockade of motor neuron K channels by 4-aminopyridine prolonged the action potential and introduced a significant delay and slowing of evoked synaptic currents, demonstrating our ability to measured altered transmitter release with this system. We conclude that the kinetics of neuromuscular synaptic currents in zebrafish is not governed by receptor block.
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