期刊
JOURNAL OF VIROLOGY
卷 79, 期 17, 页码 11541-11546出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.79.17.11541-11546.2005
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资金
- NIAID NIH HHS [P30 AI042845, AI37475, AI32890, R01 AI037475, T32AI07349-15, R01 AI032890, AI042845, R37 AI037475] Funding Source: Medline
In an in vitro assay employing reconstituted nuclei, importin 7 (IPO7) has been implicated in nuclear translocation of human immunodeficiency virus type 1 (HIV-1) cDNA. Using RNA interference technology, we inhibited expression of IPO7 by 80 to 95% in primary macrophages and in HeLa cells and monitored their ability to support HIV-1 and simian immunodeficiency virus (SIV) cDNA synthesis, nuclear translocation, and infection efficiency. Marked IPO7 deficiency did not alter the rate or extent of HIV-1 or SIV cDNA synthesis or nuclear translocation. The infection efficiency of HIV-1 was similarly unaltered. Therefore, in natural, nondividing targets of HIV-1, IPO7 may be dispensable for infection.
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