期刊
APOPTOSIS
卷 14, 期 9, 页码 1061-1069出版社
SPRINGER
DOI: 10.1007/s10495-009-0382-2
关键词
Intermedin; Myocardium; Ischemia/reperfusion; Apoptosis; Akt/GSK-3 beta
资金
- National Natural Science Foundation of China [30770869, 30871013]
- Major Basic Research Development Program of the People's Republic of China [2006CB503807]
Intermedin (IMD) is a novel member of the calcitonin/calcitonin gene-related peptide family. We investigated the cardioprotective mechanism of IMD1-53 in the in vivo rat model of myocardial ischemia/reperfusion (I/R) injury and in vitro primary neonatal cardiomyocyte model of hypoxia/reoxygenation (H/R). Myocardial infarct size was measured by 2,3,5-triphenyl tetrazolium chloride staining. Cardiomyocyte viability was determined by trypan blue staining, cell injury by lactate dehydrogenase (LDH) leakage, and cardiomyocyte apoptosis by terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling assay, Hoechst staining, gel electrophoresis and caspase 3 activity. The translocation of mitochondrial cytochrome c of myocardia and expression of apoptosis-related factors Bcl-2 and Bax, phosphorylated Akt and phosphorylated GSK-3 beta were determined by western blot analysis. IMD1-53 (20 nmol/kg) limited the myocardial infarct size in rats with I/R; the infarct size was decreased by 54%, the apoptotic index by 30%, and caspase 3 activity by 32%; and the translocation of cytochrome c from mitochondria to cytosol was attenuated. IMD1-53 increased the mRNA and protein expression of Bcl-2 and ratio of Bcl-2 to Bax by 81 and 261%, respectively. IMD1-53 (1 x 10(-7) mol/L) inhibited the H/R effect in cardiomyocytes by reducing cell death by 43% and LDH leakage by 16%; diminishing cellular apoptosis; decreasing caspase 3 activity by 50%; and increasing the phosphorylated Akt and GSK-3 beta by 41 and 90%, respectively. The cytoprotection of IMD1-53 was abolished with LY294002, a PI3K inhibitor. In conclusion, IMD1-53 exerts cardioprotective effect against myocardial I/R injury through the activation of the Akt/GSK-3 beta signaling pathway to inhibit mitochondria-mediated myocardial apoptosis.
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