期刊
DEVELOPMENTAL DYNAMICS
卷 234, 期 1, 页码 74-89出版社
WILEY
DOI: 10.1002/dvdy.20514
关键词
distal limb development; gene expression; transcriptome; microarray
资金
- NCI NIH HHS [P30 CA69533] Funding Source: Medline
To gain insight into the coordination of gene expression profiles during forelimb and hindlimb differentiation, a transcriptome analysis of mouse embryonic autopod tissues was performed using Affymetrix Murine Gene Chips (MOE-430). Forty-four transcripts with expression differences higher than 2-fold (T test, P <= 0.05) were detected between forelimb and hindlimb tissues including 38 new transcripts such as Rdh10, Frzb, Tbx18, and Hip that exhibit differential limb expression. A comparison of gene expression profiles in the forelimb, hindlimb, and brain revealed 24 limb-signature genes whose expression was significantly enriched in limb autopod versus brain tissue (fold change > 2, P <= 0.05). Interestingly, the genes exhibiting enrichment in the developing autopod also segregated into significant fore- and hindlimb-specific clusters (P <= 0.05) suggesting that by E 12.5, unique gene combinations are being used during the differentiation of each autopod type. (c) 2005 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据