期刊
BRITISH JOURNAL OF HAEMATOLOGY
卷 130, 期 5, 页码 700-708出版社
WILEY
DOI: 10.1111/j.1365-2141.2005.05664.x
关键词
multiple myeloma SET domain (MMSET); fibroblast growth factor receptor 3 (FGFR3); inhibitor of differentiation-1 (ID-1); multiple myeloma; microarray
类别
The frequently detected t(4;14)(p16.3;q32) translocation in multiple myeloma (MM) results in a dysregulation of two potential oncogenes: multiple myeloma SET domain (MMSET) and fibroblast growth factor receptor 3 (FGFR3). As the expression of FGFR3 is undetectable in 30% of the t(4;14)(+) MM patients, MMSET has been suggested to play an important role in the malignant transformation associated with the t(4;14) translocation. Screening with a real-time polymerase chain reaction (PCR) found complex expression patterns of the MMSET transcripts in fluorescence-activated cell sorted (FACS)-purified plasma cells (PCs) from 15 t(4;14)(+) MM patients. In addition, potential target genes of MMSET type I and 11 were identified, using microarray analyses of MMSET transfected cell lines. Subsequently, the expression of potential target genes was verified by real-time PCR in FACS-purified PCs from 15 t(4;14)(+) and 22 t(4;14)(-) MM patients. We suggest that the inhibitor of differentiation 1 (ID-1) is a target gene of MMSET, based on its upregulation in MMSET transfected cell lines and a significant association between the t(4;14) translocation and ID-1 expression in MM patients (P = 0.002). As high levels of ID-1 are associated with cancer, our findings indicate that MMSET promotes oncogenic transformation in t(4;14)(+) MM patients by transcriptional activation of ID-1 expression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据