期刊
PLANT AND CELL PHYSIOLOGY
卷 46, 期 9, 页码 1472-1476出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pci158
关键词
Closterium; conjugation; pheromone; sexual cell division; sexual reproduction
The sex pheromone protoplast release-inducing protein (PR-IP) inducer and a sexual cell division-inducing pheromone-minus (SCD-IP-minus) that mediates the sexual reproduction of the heterothallic Closterium peracerosum-strigosum-littorale (C. psl) complex were investigated in this study. Recombinant PR-IP inducer produced by yeast cells was prepared and assayed for production of PR-IP and induction of SCD. Both biological activities were observed after treating mating-type plus (mt(+)) cells with the recombinant pheromone. SCD was induced by exposure to a lower concentration of the same pheromone and by a shorter treatment period with the pheromone than was production of PR-IP. This indicates that the previously characterized PR-IP inducer has both PR-IP-inducing and SCD-inducing activities with mt(+) cells, although the inducing mechanisms of the two pheromones differ.
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